生物质炭对人工林土壤微生物群落和碳源可利用性的影响:室内实验毕业论文_生态学毕业论文

生物质炭对人工林土壤微生物群落和碳源可利用性的影响:室内实验毕业论文

2021-04-27更新

摘 要

生物质炭具有固碳减排的潜在作用。本研究以东台4年生杨树人工林土壤为研究对象,以水稻植株为原料,制备300和500 °C生物质炭,通过60 d的室内培养实验,设计CK(不添加生物质炭的对照)、B300(添加300°C生物质炭)和B500(添加500°C生物质炭)3个处理,利用PLFAs技术研究不同制备温度生物质炭对土壤微生物群落结构的影响。与CK处理相比,培养30 d时,B300和B500处理的G/G 菌分别提高了29.8%(p lt; 0.05)和70.0%(p lt; 0.05)。培养过程中,好氧菌/厌氧菌与G/G 菌呈现相似的变化规律。培养1 d时B300和B500处理的真菌比例分别升高了9.09%(p lt; 0.05)和9.40%(p lt; 0.05);培养60 d时2个处理的真菌比例则分别降低了30.2%(p lt; 0.05)和6.53%(p gt; 0.05)。与之相反,培养1 d时B300和B500处理的放线菌比例显著降低了21.7%(p lt; 0.05)和18.4%(p lt; 0.05);培养60 d时2个处理的放线菌比例分别升高了84.8%(p lt; 0.05)和25.1%(p gt; 0.05)。PCA分析表明,培养1、7和30 d时,生物质炭显著改变了土壤微生物群落结构(p lt; 0.05),并且3个处理的土壤微生物群落结构均随培养时间发生显著变化(p lt; 0.05)。因此,生物质炭能够通过对土壤真菌和放线菌相对丰度的改变而显著改变土壤微生物群落结构,并且这种影响随生物质炭制备温度的升高而降低。本研究结果将为深入生物质炭固碳功能提供基础数据。

关键词:生物质炭;杨树人工林;真菌;放线菌;PLFAs

Effect of Biochars Pyrolyzed at Different Temperatures on Soil Microbial Community Structure from a Poplar Plantation

ABSTRACT

Biochar is considered to be a potential measure to reduce carbon emission and thus mitigate global warming. Rice plant was used as raw material and pyrolyzed at 300 and 500 °C to produce biochars. In order to study the effect of biochars pyrolyzed at different temperatures on soil microbial community structure, a laboratory incubation of 60 days was carried out using the soil from a four-year old poplar plantation in Dongtai forest farm, Yancheng City, Jiangsu Province. The incubation included three treatments: soil without biochar amendment (CK), soil amended with biochar pyrolyzed at 300 ºC (B300), and soil amended with biochar pyrolyzed at 500 ºC (B500), and phospholipid fatty acids (PLFAs) was used to estimate soil microbial community structure. Compared with CK, the ratio of G bacteria to G bacteria increased by 29.8% (p lt; 0.05) and 70.0% (p lt; 0.05) on the 30th day in B300 and B500 treatments, respectively. During the incubation, the ratio of aerobes to anaerobes showed similar pattern with that of the ratio of G bacteria to G bacteria. Compared with CK, the percentage of fungi increased by 9.09% (p lt; 0.05) and 9.40% (p lt; 0.05) on the 1st day, while it decreased by 30.2% (p lt; 0.05) and 6.53% (p gt; 0.05) on the 60th day in B300 and B500 treatments, respectively. In contrast, the percentage of actinomycetes significantly decreased by 21.7% (p lt; 0.05) and 18.4% (p lt; 0.05) on the 1st day, while it increased by 84.8% (p lt; 0.05) and 25.1% (p gt; 0.05) on the 60th day in B300 and B500 treatments, respectively. PCA analysis showed that biochar significantly changed the soil microbial community structure (p lt; 0.05) on the 1st, 7th and 30th days of incubation, and soil microbial community structure significantly changed with incubation time in CK, B300 and B500 treatments (p lt; 0.05). Therefore, biochar can significantly change the microbial community structure, mainly due to the changes in the relative abundance of soil fungi and actinomycetes, and the effects increase with the pyrolized temperature of biochar in the poplar plantation soil. The results of this study could provide basic data for further study of carbon sequestration by biochar.

Key words: Biochar; Poplar plantation; Fungi; Actinomycetes; PLFAs

目 录

1文献综述…………………………………………………………………………………………………1

1.1生物质炭及其性质…………………………………………………………………………………1

1.1.1生物质炭………………………………………………………………………………………1

1.1.2生物质炭的性质………………………………………………………………………………1

1.2生物质炭对土壤微生物群落结构的影响…………………………………………………………1

1.2.1土壤真菌和放线菌……………………………………………………………………………2

1.2.2土壤G和G 菌………………………………………………………………………………2

1.3生物质炭影响土壤微生物群落结构的原因………………………………………………………5

2研究背景、内容和技术路线………………………………………………………………………………6

2.1研究背景……………………………………………………………………………………………6

2.2研究内容……………………………………………………………………………………………6

2.3技术路线……………………………………………………………………………………………7

3材料与方法………………………………………………………………………………………………8

3.1研究区概况…………………………………………………………………………………………8

3.2土壤样品采集………………………………………………………………………………………8

3.3生物质炭制备………………………………………………………………………………………8

3.4室内培养……………………………………………………………………………………………9

3.5土壤微生物群落结构测定…………………………………………………………………………9

3.6数据处理……………………………………………………………………………………………10

以上是资料介绍,完整资料请联系客服购买,微信号:bysjorg 、QQ号:3236353895

群聊信息

  • 还没有任何群聊信息,你来说两句吧

推荐链接