淡竹耐盐碱特性研究毕业论文_生态学毕业论文

淡竹耐盐碱特性研究毕业论文

2021-04-27更新

摘 要

盐碱土是陆地上分布广泛的一种土壤类型,是限制农业生产的一个重要因素。竹子是重要的森林资源,具有抗盐碱、生长快、成材早、产量高、用途广等优点。淡竹(Phyllostachys glauca)是我国重要竹种之一,自然分布很广,对气候适应范围大。本实验以淡竹这一重要的林业树种为材料测定淡竹在不同的盐碱胁迫条件下的生理生化反应,通过测定丙二醇含量,POD、SOD含量,脯氨酸含量,叶绿素含量,相对电导率等抗性指标来评价淡竹的耐盐碱性,主要研究结果如下:

(1)淡竹叶片中的 SOD 、POD活性均随盐浓度呈现“升-降”的变化趋势,POD酶活性随盐处理浓度及处理时间的变化与SOD酶活性的变化趋势基本一致。淡竹的保护酶SOD、POD在抗盐的过程中起到协同保护以适应盐碱胁迫的作用。

(2)低盐和中盐对应的MDA含量变化比较平缓,MDA含量没有随时间的延长发生明显的上升趋势,尽管淡竹体内可能存在胁迫诱导产生的自由基伤害,但能够通过一定的自我调节控制降低盐分胁迫所引发的膜脂过氧化作用。

(3)本实验中,随着盐碱胁迫浓度的增加和胁迫时间的延长,植物叶片叶绿素a、b含量和叶绿素总量均呈有规律的递减,而叶绿素a/b比值呈逐渐上升趋势。

(4)淡竹受到盐碱胁迫后脯氨酸含量变化明显,尤其是高盐处理下脯氨酸含量迅速升高下降,说明淡竹在处理初期通过加快脯氨酸合成的速度来提高自身渗透调节功能,而持续的胁迫下淡竹已经不能适应盐碱胁迫,生长受到抑制,植物细胞受到的伤害严重。

关键词:淡竹;盐碱胁迫;生理生化特性

Studies on Stress Tolerance of Phyllostachys glauca Under Saline-alkaline Stress

ABSTRACT

Saline-alkaline soil is one of widely distributed soil types of the land, is an important factor to limit the agricultural production. Bamboo is the important forest resources, it has many advantages, such as the saline-alkaline resistance, fast growth, early lumber, high yield, wide application. Henon bamboo(Phyllostachys glauca) is an important bamboo type with wide adaptation and distributes extensively in China. The physiological-biochemical index of POD, SOD, proline, chlorophyll content and relative conductivity were measured to evaluate the salt resistance ability of henon bamboo. The main research result is as follows:

(1) Studied on the effects of salt stresses on protective enzyme of Phyllostachys glauca. At the early stage of neutral salt stress, the activities of SOD and POD were increased and the damage of free radical was off set. At later stage of the stresses, the decreasing of SOD and POD activities indicated that the capacity of eliminating free radical was weakening. Under the stress of the saline-alkaline salt, the change law of the SOD and POD activities was the same to the neutral salt stress.

(2) Under the stress of medium and low salt stage, the content of MDA didn`t change obviously. It is because Phyllostachys glaucathe can reduce the salt stress membrane lipid per oxidation of leaves through certain self adjusting control.

(3) In this study, with the increasing of salt concentration, the general trend of Chlorophyll a and b was decreased, and the Chlorophyll a/b of Phyllostachys glauca leaves appeared an increasing trend.

(4) Under saline-alkaline stress, the pro content of Phyllostachys glaucaas increased obviously, especially at the high stage. In the early period, Phyllostachys glaucaas improve synthesis of osmotic regulation functions by increasing the speed of proline. As the stress continues, the bamboo is hurt more and more,the growth is already restrained and can not adapt to stress any more .

Key words:Phyllostachys glauca;saline-alkaline stress;physiological-biochemical

characteristic;

目 录

1 引言…………………………………………………………………………………………1

1.1 研究目的与意义 …………………………………………………………………………1

1.2 国内外研究现状 …………………………………………………………………………2

1.2.1 植物的耐盐机制 ……………………………………………………………………2

1.2.2 竹类植物的盐碱胁迫研究进展 ………………………………………………………3

2 材料与方法 ………………………………………………………………………………4

2.1 试验材料 ………………………………………………………………………………4

2.2 试验设计 ……………………………………………………………………………4

2.2.1 盐碱梯度设计 ……………………………………………………………………………4

2.2.2 试验材料处理 ……………………………………………………………………………4

2.3 研究方法 ……………………………………………………………………………4

2.3.1 酶液的制取 ……………………………………………………………………………5

2.3.2 保护酶活性的测定 ………………………………………………………………………5

2.3.2.1 SOD活性的测定… ……………………………………………………………5

2.3.2.2 POD活性的测定 ………………………………………………………………5

2.3.2.3 MDA活性的测定 ………………………………………………………………5

2.3.3 脯氨酸的测定 …………………………………………………………………………6

2.3.4 叶绿素含量的测定 ……………………………………………………………………6

2.3.5 电导率的测定 …………………………………………………………………………6 3 结果与分析 ……………………………………………………………………………………………7

3.1 不同盐碱胁迫对淡竹SOD含量的影响 ………………………………………………………7

3.2 不同盐碱胁迫对淡竹POD含量的影响 ………………………………………………………9

3.3 不同盐碱胁迫对淡竹MDA含量的影响 ………………………………………………………11

3.4 不同盐碱胁迫对淡竹脯氨酸含量的影响 ……………………………………………………13

3.5 不同盐碱浓度对淡竹叶绿素含量的影响 …………………………………………………15

3.6 不同盐碱浓度对淡竹电导率的影响 ……………………………………………………………20

结论 ……………………………………………………………………………………………………23

致谢 ………………………………………………………………………………………………………24

参考文献 …………………………………………………………………………………………………25

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