毛金竹的耐盐碱特性研究毕业论文_生态学毕业论文

毛金竹的耐盐碱特性研究毕业论文

2021-04-27更新

摘 要

土壤盐碱问题作为一个全球性的环境问题,已经成为国际社会关注的焦点。人们越来越重视研究植物对不同盐碱胁迫的反应和适应机理,以减轻盐碱土壤给农林业带来的损失。竹子是重要的森林资源,具有抗盐碱、分布广、生长快、用途多等特点,被誉为“绿色的金矿”,因此,选用竹子作为抗盐碱的研究对象不仅具有重大的理论意义,还将产生巨大的经济和社会效益。本文以毛金竹作为试材,通过不同盐碱土配比梯度的土壤基质栽培试验,研究不同盐碱胁迫条件下毛金竹的叶绿素含量、脯氨酸含量、丙二醛含量和SOD、POD酶的活性等抗性生理生化指标的动态变化,以揭示毛金竹的耐盐碱特性。

主要结果如下:

  1. 在胁迫的不同时期,随着盐分胁迫浓度的增加,毛金竹叶片叶绿素a、b和总量均呈逐渐减少趋势,且低于对照,说明盐碱胁迫下毛金竹的光合性能受到影响。
  2. 在200mM的Na2CO3处理组内,毛金竹的脯氨酸含量下降速度有明显增长,说明碱胁迫浓度越大,对毛金竹的生长抑制越明显。
  3. 随着胁迫时间的延长,SOD活性呈现“升高-下降-升高”的趋势,在胁迫第30天达到最大值;POD呈现“下降-升高”的趋势,在第30天达到最低值。说明持续的盐碱胁迫对毛金竹产生了明显的伤害作用,毛金竹抗氧化能力衰退。

4. 盐碱胁迫处理过程中,毛金竹MDA含量变化不大,且与对照相比无显著差异,说明碱胁迫对毛金竹体内膜系统伤害不大。

关键词:毛金竹;盐碱胁迫;生理指标;耐盐碱性

Study on Saline-alkali Tolerance of Phyllostachys nigra

ABSTRACT

The problem of soil salinization has already become the focus that international community has paid close attention to as a global environmental problem. People have put more and more efforts on studying the mechanism of reaction and adaptation to different saline-alkali stress of plants, so that they can protect the agroforestry from saline soil’s damage. Bamboo are important forest resources with many advantages, such as the saline-alkaline resistance, high yield, fast growth, wide application, known as “greengold.” So taking bamboo as the research object of saline-alkaline resistance not only has important theoretical significance, but also can bring huge economic and social benefits. Taking Phyllostachys nigra as the major test material, through different treatments of saline stress, the changes of the growth and some stress-resistant physiological and biochemical indexes were studied in this paper, such as the content of chlorophyll, praline and MDA and activity of SOD and POD, so that the saline-alkali tolerance of Phyllostachys nigra can be discovered. The main results are shown as follows.

1. In different stages of the stress, the Chlorophyll a, b and Chlorophyll(a b) contents all decreased with the increasing concentration of salt stress and were lower than the CK, which means the photosynthesis of Phyllostachys nigra were affected under the saline-alkali stress.

2. Under the 200mM Na2CO3 stress, the drop speed of proline content increased obviously, which means the inhibitory effect on the growth increased with the concentration of the saline-alkali stress.

3. As the stress lasted, the SOD activity showed an “up-down-up” trend and reached a maximum value on the 30th of the stress, which means the sustained saline-alkali stress caused obvious damage to the antioxidant abilities of Phyllostachys nigra.

4. In the process of the saline-alkali stress, the MDA content showed little change, as well as little difference between the CK, which means the saline-alkali stress hardly caused damage to the membrane systems of Phyllostachys nigra.

Key words: Phyllostachys nigra; saline-alkali stress; physiological indieators; saline-alkali tolerance

目 录

1 绪论………………………………………………………………………………………………………1

1.1 植物耐盐碱研究概述…………………………………………………………………………1

1.2 盐碱胁迫对植物生理指标的影响 ……………………………………………………………3

1.3 毛金竹概况及开发利用…………………………………………………………………………4

1.4 研究目的及意义…………………………………………………………………………………4

2 材料与方法………………………………………………………………………………………………5

2.1 试验材料…………………………………………………………………………………………5

2.2 试验方法…………………………………………………………………………………………6

2.2.1 酶液提取……………………………………………………………………………………6

2.2.2 毛金竹各生理生化指标测定………………………………………………………………6

2.2.2.1 叶绿素的测定……………………………………………………………………………6

2.2.2.2 脯氨酸的测定……………………………………………………………………………7

2.2.2.3 MDA的测定……………………………………………………………………………7

2.2.2.4 POD的测定………………………………………………………………………………7

2.2.2.5 SOD的测定………………………………………………………………………………7

3 结果与分析………………………………………………………………………………………………8

3.1 盐碱胁迫对毛金竹叶绿素的影响………………………………………………………………8

3.2 盐碱胁迫对毛金竹脯氨酸的影响………………………………………………………………15

3.3 盐碱胁迫对毛金竹MDA的影响………………………………………………………………18

3.4 盐碱胁迫对毛金竹两种保护酶活性的影响……………………………………………………20

3.4.1 盐碱胁迫对毛金竹POD的影响…………………………………………………………20

3.4.2 盐碱胁迫对毛金竹SOD的影响…………………………………………………………23

结论…………………………………………………………………………………………………………25

致谢…………………………………………………………………………………………………………26

参考文献……………………………………………………………………………………………………27

1 绪论

近年来,由于环境恶化,土壤污染和土壤退化的问题日益严重,土壤盐渍化成为全球性环境焦点问题。当前,全球盐碱地分布面积达到陆地面积的7.6%,土地盐渍化已严重影响到农林业生产与生态环境。据联合国环境规划署(UNEP)的调查和我国农业部组织的第二次全国土壤普查资料统计,因土壤盐渍化造成的土地荒漠化达110万hm²以上,仅次于风蚀和水蚀,居第三位[1]。据不完全统计,全世界的盐渍土面积有9.5×108hm²,中国约有2.7×107hm²。在中国0.67×108hm² 耕地中就有10%为盐渍化土壤[2]。我国目前尚有部分潜在盐渍化土壤,若开发利用措施不当,极易发生盐渍化。土壤盐渍化的生态破坏性极其严重,对人类生存的危害也日益加重。

目前针对盐分对植物的伤害以及植物对盐碱环境的适应机理的研究越来越受到学者们的重视,众多国内外学者在对盐碱胁迫下树木生长发育、生理生化反应的研究以及基因工程在树木抗盐碱方面的运用等方面做了大量研究。国内外对林木的耐盐碱性研究主要集中于根、茎、叶的生长表型,以及包括水分代谢、细胞膜透性、渗透调节、光合生理、活性氧代谢、植物激素等在内的生理生化方面,并取得了一定的进展。人们越来越重视研究植物对不同盐碱浓度环境条件的反应和适应机理,以减轻盐碱土壤给农林业带来的伤害和损失。

以上是资料介绍,完整资料请联系客服购买,微信号:bysjorg 、QQ号:3236353895

群聊信息

  • 还没有任何群聊信息,你来说两句吧

推荐链接